Journal: Nucleic Acids Research

Article Title: E. coli RsmF activity depends on prior modification of 16S rRNA helix 44

doi: 10.1093/nar/gkag1005

Figure Lengend Snippet: A cluster of rRNA modifications in h44 surrounds the ribosome decoding center on the 30S subunit. ( A ) Structure of the Escherichia coli 30S subunit (PDB 8FTO) with a zoomed-in view of the top of h44 and the neighboring 16S rRNA helices that form the A and P sites. ( B ) Detailed views of the structural context of each h44 methylated nucleotide m 3 U1498 (left), m 4 Cm1402 (center), and m 5 C1407 (right). The h44 methyl groups are shown as colored-coded spheres in panels A and B. ( C ) Workflow for generation of double, triple, and quadruple Rsm deletion strains. Solid line arrows denote connections from recipient strain to new deletion, and dashed line arrows indicate the DNA source for P1 phage transduction.

Article Snippet: Libraries were then constructed with the Nextera XT DNA library Preparation Kit (Illumina, FC-131-1096) and Illumina DNA/RNA UD indexes at the Emory NPRC Genomics Core.

Techniques: Methylation, Transduction